Journal: Cell Transplantation

Article Title: HBO Promotes the Differentiation of Neural Stem Cells via Interactions Between the Wnt3/β-Catenin and BMP2 Signaling Pathways

doi: 10.1177/0963689719883578

Figure Lengend Snippet: Expression of Wnt3a, BMP2 and β-catenin nuclear proteins in NSCs after HBO treatment by Western blotting. (A) Expression of Wnt3a and BMP2 protein: HBO treatment up-regulated the expression of Wnt3a and BMP2 proteins. (B) Expression of β-catenin nuclear and cytoplasmic proteins: HBO up-regulated the expression of β-catenin nuclear protein; however, the expression of β-catenin cytoplasmic protein was not affected. (C and D) Each bar represents mean±SD, n = 3. a p < 0.05 vs. the CON group, ANOVA test; b p < 0.05 vs. the HBO group, ANOVA test; c p < 0.05 vs. the NBH group, ANOVA test; d p < 0.05 vs. the HIBH group, ANOVA test. NSCs: neural stem cells; HBO: hyperbaric oxygen; CON: control; NBH: normal brain tissue extracts; HIBH: hypoxic-ischemic brain damage tissue extracts; BMP2: bone morphogenetic protein.

Article Snippet: The following primary antibodies were used: Rabbit mAb active Wnt3 (1:100; SC-28824, Santa Cruz, Biotechnology, Inc., Dallas, TX, USA), rabbit mAb active β-catenin (1:100; BA0426, Wuhan Boster Biological Technology, Ltd.), rabbit mAb active BMP2 (1:100; PB0727, Wuhan Boster Biological Technology, Ltd.), mouse mAb β-actin (1:200, BM0626, Wuhan Boster Biological Technology, Ltd.) and mouse mAb active PCNA (1:1000; 610664, BD Biosciences, San Jose, CA, USA).

Techniques: Expressing, Western Blot, Control

Journal: Cell Transplantation

Article Title: HBO Promotes the Differentiation of Neural Stem Cells via Interactions Between the Wnt3/β-Catenin and BMP2 Signaling Pathways

doi: 10.1177/0963689719883578

Figure Lengend Snippet: Expression, after HBO treatment, of Wnt3a, BMP2 and β-catenin nuclear proteins in the presence of sFRP2/3 was determined by Western blotting. (A) Expression of Wnt3a and BMP2 proteins: sFRP2/3 reduced the expression of Wnt3a and BMP2 proteins after HBO treatment. (B) Expression of β-catenin nuclear and cytoplasmic proteins: sFRP2/3 reduced the expression of β-catenin nuclear protein after HBO treatment; the expression of β-catenin cytoplasmic protein was not affected. (C and D) Each bar represents mean±SD, n = 3. a p < 0.05 vs. the HIBD+HBO group, ANOVA test; b p < 0.05 vs. the HBO group, ANOVA test. HBO: hyperbaric oxygen; CON: control; HIBD: hypoxic-ischemic brain damage; sFRP2/3: secreted Frizzled-related protein 2 and 3; BMP2: bone morphogenetic protein.

Article Snippet: The following primary antibodies were used: Rabbit mAb active Wnt3 (1:100; SC-28824, Santa Cruz, Biotechnology, Inc., Dallas, TX, USA), rabbit mAb active β-catenin (1:100; BA0426, Wuhan Boster Biological Technology, Ltd.), rabbit mAb active BMP2 (1:100; PB0727, Wuhan Boster Biological Technology, Ltd.), mouse mAb β-actin (1:200, BM0626, Wuhan Boster Biological Technology, Ltd.) and mouse mAb active PCNA (1:1000; 610664, BD Biosciences, San Jose, CA, USA).

Techniques: Expressing, Western Blot, Control

Journal: European Journal of Medical Research

Article Title: The TEAD4-DYNLL1 axis accelerates cell cycle progression and augments malignant properties of lung adenocarcinoma cells

doi: 10.1186/s40001-025-02500-y

Figure Lengend Snippet: Silencing of DYNLL1 reduces malignant phenotype of LUAD cells and blocks cell cycle progression. Three KD-DYNLL1 plasmids (KD-DYNLL1 1#, 2#, 3#) were transfected into NCI-H1395 and NCI-H441 cells using lentiviral vectors. A DYNLL1 mRNA levels in transfected cells were measured by qPCR, with KD-DYNLL1 2# exhibiting the strongest knockdown effect, selected for subsequent experiments. B Colony formation assay to evaluate the colony-forming ability of KD-DYNLL1-transfected cells. C Immunofluorescence staining for Ki67 to assess cell proliferation potential. D Flow cytometry analysis of cell apoptosis. E Wound healing assays to evaluate cell migration. F Transwell assay to assess cell invasion. G Flow cytometry analysis of cell cycle distribution. H Western blot analysis of protein expression levels of Cyclin D1, p21, and β-catenin in transfected cells. Each dot corresponds to one independent experiment. Differences were analyzed by two-way ANOVA ( A – H ) * p < 0.05, ** p < 0.01, **** p < 0.0001

Article Snippet: Following blocking with 5% skim milk, these membranes were probed with antibodies for DYNLL1 (1:1000, ab51603, Abcam), Cyclin D1 (1:200, ab16663, Abcam), P21 (1:1000, MA5-31479, Thermo Fisher), β-catenin (1:100, A11512, Abclonal), TEAD4 (1:1000, 12418-1-AP, Proteintech Group, Wuhan, Hubei, China), GAPDH (1:5000, 10494-1-AP, Proteintech) overnight.

Techniques: Transfection, Knockdown, Colony Assay, Immunofluorescence, Staining, Flow Cytometry, Migration, Transwell Assay, Western Blot, Expressing

Journal: European Journal of Medical Research

Article Title: The TEAD4-DYNLL1 axis accelerates cell cycle progression and augments malignant properties of lung adenocarcinoma cells

doi: 10.1186/s40001-025-02500-y

Figure Lengend Snippet: DYNLL1 overexpression activates the Wnt/β-catenin pathway and rescues malignant properties of LUAD cells suppressed by TEAD4 knockdown. NCI-1395 and NCI-H441 cells transfected with KD-TEAD4 were additionally administered OE-DYNLL1. A DYNLL1 mRNA expression in cells measured by qPCR analysis. B Colony formation assays to evaluate the colony-forming ability of treated cells. C Immunofluorescence staining for Ki67 to assess cell proliferation. D Flow cytometry analysis of cell apoptosis. E Flow cytometry analysis of cell cycle distribution. F SA-β-Gal (SABG) staining to analyze cell senescence. G Western blot (WB) analysis of Cyclin D1 and p21 protein levels. Each dot corresponds to one independent experiment. Differences were analyzed by two-way ANOVA ( A – G ). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: Following blocking with 5% skim milk, these membranes were probed with antibodies for DYNLL1 (1:1000, ab51603, Abcam), Cyclin D1 (1:200, ab16663, Abcam), P21 (1:1000, MA5-31479, Thermo Fisher), β-catenin (1:100, A11512, Abclonal), TEAD4 (1:1000, 12418-1-AP, Proteintech Group, Wuhan, Hubei, China), GAPDH (1:5000, 10494-1-AP, Proteintech) overnight.

Techniques: Over Expression, Knockdown, Transfection, Expressing, Immunofluorescence, Staining, Flow Cytometry, Western Blot

Journal: European Journal of Medical Research

Article Title: The TEAD4-DYNLL1 axis accelerates cell cycle progression and augments malignant properties of lung adenocarcinoma cells

doi: 10.1186/s40001-025-02500-y

Figure Lengend Snippet: TEAD4 and DYNLL1 affects tumorigenesis of LA795 cells in mice. Mouse LUAD cells (LA795; 5 × 10 6 cells per mouse) stably transfected with KD-TEAD4 and OE-DYNLL1 were implanted into BALB/c mice to induce allograft tumors. A Tumor volume measured over a 5-week period. B Representative images of tumors and tumor weight after 5 weeks of growth. C Western blot (WB) analysis for the expression of β-catenin, TEAD4, and DYNLL1 in tumor tissues. D Immunohistochemistry (IHC) for the positive staining of proliferation markers Ki67 and PCNA in tumor tissues. E IHC for the positive staining of Cyclin D1 and p21 levels in tumor tissues. F TUNEL assays to detect cell apoptosis within the tumor tissues. Differences were analyzed by two-way ANOVA ( A – F ). Each group contained six mice. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: Following blocking with 5% skim milk, these membranes were probed with antibodies for DYNLL1 (1:1000, ab51603, Abcam), Cyclin D1 (1:200, ab16663, Abcam), P21 (1:1000, MA5-31479, Thermo Fisher), β-catenin (1:100, A11512, Abclonal), TEAD4 (1:1000, 12418-1-AP, Proteintech Group, Wuhan, Hubei, China), GAPDH (1:5000, 10494-1-AP, Proteintech) overnight.

Techniques: Stable Transfection, Transfection, Western Blot, Expressing, Immunohistochemistry, Staining, TUNEL Assay

Journal: European Journal of Medical Research

Article Title: The TEAD4-DYNLL1 axis accelerates cell cycle progression and augments malignant properties of lung adenocarcinoma cells

doi: 10.1186/s40001-025-02500-y

Figure Lengend Snippet: Graphic Abstract. TEAD4 regulates DYNLL1 transcription to activate the Wnt/β-catenin signaling, thus promoting cell cycle progression and the malignant properties of LUAD cells

Article Snippet: Following blocking with 5% skim milk, these membranes were probed with antibodies for DYNLL1 (1:1000, ab51603, Abcam), Cyclin D1 (1:200, ab16663, Abcam), P21 (1:1000, MA5-31479, Thermo Fisher), β-catenin (1:100, A11512, Abclonal), TEAD4 (1:1000, 12418-1-AP, Proteintech Group, Wuhan, Hubei, China), GAPDH (1:5000, 10494-1-AP, Proteintech) overnight.

Techniques: